Citation

Bourgogne E, Herrou V, Mathurin JC, Becchi M, de Ceaurriz J. Rapid Commun. Mass Spectrom. 2000; 14(24): 2343-2347.

Affiliation

Laboratoire National de Dépistage du Dopage, 143 avenue R. Salengro, 92290 Chatenay Malabry, France.

Copyright

(Copyright © 2000, John Wiley and Sons)

DOI

10.1002/1097-0231(20001230)14:24<2343::AID-RCM171>3.0.CO;2-Z

PMID

11114048

Abstract

A detailed procedure for the analysis of exogenous hydrocortisone and cortisone in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is proposed. As urinary levels of hydrocortisone are rather low for GC/C/IRMS analysis, the focus is on the main corticosteroid metabolites, tetrahydrocortisone (THE) and tetrahydrocortisol (THF). Following different solid phase extraction purifications, THE and THF are oxidized to 5beta-androstanetrione before analysis by GC/C/IRMS. Significant differences in delta(13)C per thousand values of synthetic natural corticosteroids and endogenous human corticosteroids have been observed. Therefore, a positive criterion, to detect exogenous administration of synthetic corticosteroids in anti-doping control, is proposed.

Language: en