Citation

Ren S, Cheng K, Song A, Zhang J. Sheng Wu Gong Cheng Xue Bao 2011; 27(4): 592-597.

Affiliation

College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.

Copyright

(Copyright © 2011, Ke xue chu ban she)

DOI

unavailable

PMID

21847994

Abstract

In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase, the effects of substrate concentration, cellulase concentration and temperature were determined. The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation, and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours. In the condition of 45 degrees C and pH 5.0 and the stirring rate 120 r/min, the Michealis constant (Km) and maximum rate (Vm) for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/(L x h). The kinetic model, including the parameters such as substrate concentration, enzymatic concentration and temperature, was suit for the hydrolysis reaction under the temperature range from 30 degrees C-50 degrees C.

Language: zh