Citation

Revtovich S, Morozova E, Kulikova V, Koval V, Anufrieva N, Nikulin A, Demidkina T. Biochimie 2020; 168: 190-197.

Copyright

(Copyright © 2020, Elsevier Publishing)

DOI

10.1016/j.biochi.2019.11.004

PMID

31711941

Abstract

Interactions of Citrobacter freundii methionine γ-lyase (MGL) with sulfoxides of typical substrates were investigated. It was found that sulfoxides are suicide substrates of the enzyme. The products of the β- and γ-elimination reactions of sulfoxides, thiosulfinates, oxidize three cysteine residues of the enzyme. Three-dimensional structures of MGL inactivated by dimethyl thiosulfinate and diethyl thiosulfinate were determined at 1.46 Å and 1.59 Å resolution. Analysis of the structures identified SH groups oxidized by thiosulfinates and revealed the structural bases of MGL inactivation. The extent of inactivation of MGL in the catalysis of the β-elimination reaction depends on the length of the "tail" at oxidized Cys115. Oxidation of Cys115 results in MGL incapable to catalyze the stage of methyl mercaptan elimination of the physiological reaction.

Language: en

Keywords

Kinetics; Citrobacter freundii; Models, Molecular; Bacterial Proteins; Cysteine; Amino Acids; Ligands; Sulfoxides; Carbon-Sulfur Lyases; Methionine γ-lyase; Structural bases of enzyme inactivation; Suicidal substrates; Sulfoxides of typical substrates