Citation

Munoz-Munoz JL, García-Molina F, Acosta-Motos JR, Arribas E, García-Ruiz PA, Tudela J, García-Cánovas F, Rodriguez-Lopez JN. Acta Biochim. Pol. 2011; 58(4): 477-488.

Copyright

(Copyright © 2011, Panstwowe Wydawnictwo Naukowe)

DOI

unavailable

PMID

22187676

Abstract

Under aerobic conditions, tyrosinase is inactivated by dopa as a result of suicide inactivation, and, under anaerobic conditions, as a result of irreversible inactivation. However, tyrosine protects the enzyme from being inactivated by dopa under anaerobic conditions. This paper describes how under aerobic conditions the enzyme acting on tyrosine is not directly inactivated but undergoes a process of indirect suicide inactivation provoked by reaction with the o-diphenol originated from the evolution of o-dopaquinone and accumulated in the reaction medium.

Language: en

Keywords

Kinetics; Oxygen; Phenols; Oxidation-Reduction; Enzyme Inhibitors; Magnetic Resonance Spectroscopy; Agaricales; Dihydroxyphenylalanine; Enzyme Assays; Tyrosine; Computer Simulation; Stereoisomerism; Models, Molecular; Enzyme Activation; Oxidoreductases; Monophenol Monooxygenase; Benzoquinones