Citation

Schicher M, Kollroser M, Hermetter A. Methods Mol. Biol. 2009; 579: 497-511.

Copyright

(Copyright © 2009, Holtzbrinck Springer Nature Publishing Group)

DOI

10.1007/978-1-60761-322-0_25

PMID

19763492

Abstract

Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl esters in animals, plants, and microorganisms. In this chapter we describe a tool for the concomitant analysis of lipases in complex proteomes. For this purpose, the target enzymes are selectively and covalently labelled with fluorescent suicide inhibitors. Stable lipid-protein complexes are formed that are separated by gel electrophoresis and identified by mass spectrometry.

Language: en

Keywords

Adipose Tissue; Alkylation; Animals; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Isoelectric Focusing; Lipase; Lipids; Mice; Oxidation-Reduction; Proteome; Tandem Mass Spectrometry