Citation

Kitamura Y, Mori K, Yamamoto M, Nozaki A, Saito M, Tsukamoto I, Mifune M, Saito Y. Chem. Pharm. Bull. (Tokyo) 2008; 56(9): 1364-1366.

Copyright

(Copyright © 2008, Pharmaceutical Society of Japan)

DOI

10.1248/cpb.56.1364

PMID

18758123

Abstract

In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activity of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn(3+)-octabromotetrakis(1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.

Language: en

Keywords

Catalysis; Enzymes, Immobilized; Horseradish Peroxidase; Ion Exchange Resins; Manganese; Metalloporphyrins; Peroxidases; Porphyrins