Citation

Motojyuku M, Saito T, Akieda K, Otsuka H, Yamamoto I, Inokuchi S. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2008; 875(2): 509-514.

Copyright

(Copyright © 2008, Elsevier Publishing)

DOI

10.1016/j.jchromb.2008.10.003

PMID

18945648

Abstract

This paper describes an assay for the determination of glyphosate (GLYP), glyphosate metabolites [(aminomethyl) phosphonic acid] (AMPA), and glufosinate (GLUF) in human serum. After protein precipitation using acetonitrile and solid-phase extraction, serum samples were derivatized and analyzed by gas chromatography-mass spectrometry (GC-MS). The assay was linear over a concentration range of 3-100.0 microg/ml for GLYP, AMPA, and GLUF. The overall recoveries for the three compounds were >73%. The intra- and inter-day variations were <15%. Precision and accuracy were 6.4-10.6% and 88.2-103.7%, respectively. The validated method was applied to quantify the GLYP and AMPA content in the serum of a GLYP-poisoned patient. In conclusion, the method was successfully applied for the determination of GLYP and its metabolite AMPA in serum obtained from patient of GLYP-poisoning.

Language: en

Keywords

Aminobutyrates; Drug Stability; Gas Chromatography-Mass Spectrometry; Glycine; Glyphosate; Herbicides; Humans; Isoxazoles; Linear Models; Male; Middle Aged; Organophosphonates; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Suicide, Attempted; Tetrazoles