Citation

Wei G, Hough CJ, Sarvey JM. Neurosci. Lett. 2004; 370(2-3): 118-122.

Copyright

(Copyright © 2004, Elsevier Publishing)

DOI

10.1016/j.neulet.2004.08.027

PMID

15488306

Abstract

3-nitropropionic acid (3-NPA), a suicide inhibitor of succinate dehydrogenase (SDH; complex II), has been used to provide useful experimental models of Huntington's disease (HD) and "chemical hypoxia" in rodents. The trace ion Zn2+ has been shown to cause neurodegeneration. Employing real-time Newport Green fluorescence imaging of extracellular Zn2+, we found that 3-NPA (10-100 microM) caused a concentration-dependent increase in the concentration of extracellular Zn2+ ([Zn2+]o) in acute rat hippocampus slices. This increase in [Zn2+]o was abolished by 10 mM CaEDTA. The increase of [Zn2+]o was also accompanied by a rapid increase of cytoplasmic-free Zn2+ concentration ([Zn2+]i). The induction of Zn2+ release by 3-MPA in hippocampus slices points to a potential mechanism by which 3-NPA might induce neurodegeneration.

Language: en

Keywords

Animals; Dose-Response Relationship, Drug; Drug Interactions; Edetic Acid; Extracellular Space; Hippocampus; In Vitro Techniques; Microscopy, Fluorescence; Nitro Compounds; Propionates; Rats; Succinate Dehydrogenase; Time Factors; Zinc