Citation

Lefèvre S, Debat H, Thomas D, Friboulet A, Avalle B. FEBS Lett. 2001; 489(1): 25-28.

Copyright

(Copyright © 2001, Federation of European Biochemical Societies, Publisher Elsevier Publishing)

DOI

10.1016/s0014-5793(01)02075-0

PMID

11231007

Abstract

The catalytic mechanism of an anti-idiotypic antibody, 9G4H9, displaying a beta-lactamase activity was investigated. Kinetics experiments suggest that some penicillinic derivatives behave both as substrates and inactivators. Biochemical and immunological experiments strongly indicate that ampicillin may be regarded as a suicide substrate for hydrolysis by 9G4H9. The anti-idiotypic network appears as a way to create enzyme mimics with modified catalytic activities.

Language: en

Keywords

Animals; Antibodies, Anti-Idiotypic; Antibodies, Catalytic; Antibodies, Monoclonal; beta-Lactams; Blotting, Western; Humans; Hydrolysis; Kinetics; Mice; Penicillins; Substrate Specificity