Citation

Chinaka S, Takayama N, Michigami Y, Ueda K. J. Chromatogr. B Biomed. Sci. Appl. 1998; 713(2): 353-359.

Copyright

(Copyright © 1998, Elsevier Publishing)

DOI

10.1016/s0378-4347(98)00176-5

PMID

9746250

Abstract

An ion chromatographic method for the simultaneous determination of cyanide and thiocyanate in blood has been developed. After extraction by adding water and methanol to blood, cyanide was derivatized with 2,3-naphthalenedialdehyde and taurine to give a fluorescent product of 1-cyanobenz[f]isoindole. This compound was detected with high sensitivity by fluorometry and the underivatized thiocyanate was detected by ultraviolet absorption. The detection limits were 3.8 pmol ml(-1) for cyanide and 86 pmol ml(-1) for thiocyanate, and the recoveries from blood were ca. 83% and ca. 100%, respectively. The proposed method was successfully applied to the analysis of both anions in blood from smokers, non-smokers and fire victims.

Language: en

Keywords

Chromatography, Ion Exchange; Cyanides; Fires; Humans; Indicators and Reagents; Naphthalenes; Potassium Cyanide; Sensitivity and Specificity; Smoking; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Suicide; Taurine; Thiocyanates