Citation

T.m.a. EK. Egypt. J. Microbiol. 1991; 26(3): 443-451.

Copyright

(Copyright © 1991)

DOI

unavailable

PMID

unavailable

Abstract

Transposon Tn 5, which confers kanamycin-neomycin resistance, was introduced into the genome of Pseudomonas putida PpG1-M1 by conjugation with Escherichia coli SM10 containing the suicide plasmid vector pSUP2021. Approximately, 1.82% of over 2200 neomycin resistant [Nmr] colonies were mutated to auxotrophy. Most auxotrophs reverted at low frequencies [<10-8] and most prototrophic revertants were neomycin sensitive. The Tn 5-induced auxotrophic mutations were mainly distributed among genes of aspartic acid group indicating specific site-distribution of Tn 5 among the genome of Pseudomonas putida PpG1-M1

Language: en