Citation

Wang C. Science in China (Scientia Sinica) Series B 1991; 34(4): 429-437.

Copyright

(Copyright © 1991)

DOI

unavailable

PMID

unavailable

Abstract

A recombinant transposon Tn5-GmSp, produced by replacing the central BglII fragment with a BamHI one of R1033, was utilized to detect Tn5 transposition inhibition in Rhizobium leguminosarum. Two types of suicide plasmids (pXS102 and pCW3/pCW30) were constructed for Tn5-GmSp transposition. The frequency of transposition in both constructs was greatly reduced in the Rhizobium strains where a resident Tn5 was already present. When pXS102 was transferred into a Tn5-containing Rhizobium strain, the transconjugants retained the deleted forms of pXS102, indicating that the failure of transposition was due to the loss of the unstable prophage Mu element. In the case of pCW3/pCW30, vectors were not observed in the transconjugants although most of them retained the antibiotic resistant markers. This result suggested the integration of Tn5-GmSp into the rhizobial host genome through recombination. © 1991.

Language: en

Keywords

R. leguminosarum; recombinant Tn5-GmSp; Tn5 in situ replacement; transposition inhibition