Citation

Tiburcio AF, Figueras X, Claparols I, Santos M, Torné JM. Plant Cell, Tissue and Organ Culture 1991; 27(1): 27-32.

Copyright

(Copyright © 1991)

DOI

10.1007/BF00048202

PMID

unavailable

Abstract

The influence of a three-month-long pretreatment with dl-alpha-difluoromethylarginine (DFMA), an irreversible suicide inhibitor of arginine decarboxylase activity (ADC; EC 4.1.1.19), on plant regeneration, protein and polyamine (PA) composition of Zea mays callus cultures has been investigated. A four-fold increase in the number of regenerated plants is obtained after pretreatment with 0.5 mM DFMA. In addition, the regeneration frequency increases 3-fold in the treated calluses and the plants regenerated from such cultures are more developed than the untreated controls. The data obtained on protein and PA contents suggest that a senescence effect is exerted on the calluses grown in the presence of DFMA. However, after DFMA removal a rejuvenation effect occurs on the calluses that may explain the improvement of morphogenic capacity. This study indicates that DFMA pretreatment can be used to increase regeneration efficiency from maize callus cultures. © 1991 Kluwer Academic Publishers.

Language: en

Keywords

putrescine; polyamines; arginine decarboxylase; inhibitors