Citation

Houze P, Chaussard J, Harry P, Pays M. J. Chromatogr. 1993; 619(2): 251-257.

Copyright

(Copyright © 1993, Elsevier Publishing)

DOI

10.1016/0378-4347(93)80114-j

PMID

8263097

Abstract

A method has been developed for the separation and measurement of ethylene glycol and three other glycols (propylene glycol, 1,3-butylene glycol and 2,3-butylene glycol) in biological samples by wide-bore column gas chromatography with a flame ionization detector. The method used 1,3-propylene glycol (1,3-propanediol) as an internal standard. The method was linear at least from 2 to 1000 micrograms/ml, with a detection limit of 1 microgram/ml. Analytical recoveries were 89-98% for the different concentrations. Precision studies showed coefficients of variation of 1.5-7.7% for the different concentrations. The assay was applied to the analysis of biological samples from two patients who had ingested ethylene glycol and/or other glycols in a suicide attempt.

Language: en

Keywords

Adult; Boronic Acids; Chromatography, Gas; Glycols; Humans; Male; Protein Denaturation; Suicide, Attempted