Citation

Houze P, de Reynies A, Baud FJ, Benatar MF, Pays M. J. Chromatogr. 1992; 574(2): 305-312.

Copyright

(Copyright © 1992, Elsevier Publishing)

DOI

10.1016/0378-4347(92)80044-q

PMID

1618964

Abstract

A method was developed for the separation and measurement of chloroquine and three metabolites (desethylchloroquine, bisdesethylchloroquine and 4-amino-7-chloroquinoline) in biological samples by ion-pair high-performance liquid chromatography with UV detection. The method uses 2,3-diaminoaphthalene as an internal standard and provides a limit of detection between 1 and 2 ng/ml for chloroquine and its metabolites. The assay was linear in the range 12.5-250 ng/ml and the analytical recovery and reproducibility were sufficient. The assay was applied to the analysis of biological samples from a patient undergoing chloroquine chemoprophylaxis and a patient who had ingested chloroquine in a suicide attempt.

Language: en

Keywords

Chloroquine; Chromatography, High Pressure Liquid; Humans; Ions; Poisoning; Reproducibility of Results; Spectrophotometry, Ultraviolet