Citation

van Rooijen N, Poppema A. J. Pharmacol. Toxicol. Methods 1992; 28(4): 217-221.

Copyright

(Copyright © 1992, Elsevier Publishing)

DOI

10.1016/1056-8719(92)90007-n

PMID

1296827

Abstract

In earlier studies, we described a technique for the "in vivo" depletion of macrophages, using liposome-encapsulated dichloromethylene bisphosphonate (C12MBP). The aim of the present study was to compare the effects on macrophages of various other liposome-delivered Ca2+ chelators: EDTA, DTPA, and EGTA. It appeared that both EDTA and DTPA as well as Ca(2+)-and Cu(2+)-EDTA complexes were also effective in the elimination of splenic macrophages when encapsulated in liposomes and intravenously injected. EGTA, a well-known and frequently used Ca2+ chelator, had no effect.

Language: en

Keywords

Animals; Chelating Agents; Clodronic Acid; Drug Carriers; Edetic Acid; Egtazic Acid; Female; Injections, Intravenous; Liposomes; Liver; Macrophages; Mice; Mice, Inbred Strains; Pentetic Acid; Spleen