Citation

Derijk R, Berkenbosch F. Am. J. Physiol. 1992; 263(6): E1092-1098.

Copyright

(Copyright © 1992, American Physiological Society)

DOI

10.1152/ajpendo.2006.263.6.E1092

PMID

1476182

Abstract

We describe the development and application of a radioimmunoassay to detect circulating interleukin (IL)-1 beta concentrations in the rat. No IL-1 immunoreactivity above the detection limit of the assay (100 pg/ml) could be detected in plasma of control rats. In contrast, immunoreactive IL-1 was detected after intravenous administration of rat recombinant IL-1 beta (rrIL-1 beta) or bacterial lipopolysaccharide (LPS) to rats. The effect of LPS on plasma immunoreactive IL-1 concentrations was time and dose dependent. The immunoreactive IL-1 response to LPS was prevented by in vivo macrophage depletion induced by liposome-directed macrophage suicide technique. Gel filtration of plasma from LPS-treated rats revealed the presence of a high and a smaller molecular form of immunoreactive IL-1. The small molecular immunoreactive IL-1 peak coeluted with rrIL-1 beta and probably represents the 17-kDa form of IL-1 beta. In conclusion, our data support the hypothesis that IL-1 secreted by macrophages can act as a humoral signal molecule to induce the immunological, metabolic, and neuroendocrine changes in response to bacterial LPS.

Language: en

Keywords

Animals; Antibodies; Chromatography; Humans; Interleukin-1; Lipopolysaccharides; Macrophages; Radioimmunoassay; Rats; Recombinant Proteins; Titrimetry