Citation

Chattopadhyay D, Lodha ML. J. Plant Biochem. Biotechnol. 1993; 2(1): 15-18.

Copyright

(Copyright © 1993, Holtzbrinck Springer-Nature)

DOI

10.1007/BF03262915

PMID

unavailable

Abstract

Transposon Tn5 mutants of two Cicer-Rhizobium strains, G36-84 and F-75 tolerant to nitrate under symbiotic condition, have been developed. Suicide vector pGS9 was used as a source of Tn5 for insertion mutagenesis of Rhizobium. The mutants so developed were screened in the presence of nitrate under symbiotic condition for nodule formation (Nod) and N2-fixation (Fix) ability. We could get some mutants, which were Nod+ Fix+ at 1mM and 2 mM nitrate, from the parental strains which were Nod- at 1 mM nitrate. © 1993 Springer-Verlag.

Language: en

Keywords

Cicer-Rhizobium; nitrate tolerance; Tn5 mutants; transposon Tn5