Citation

Uchiumi T, Higashi S, Abe M. Journal of General Microbiology 1993; 139(10): 2371-2377.

Copyright

(Copyright © 1993)

DOI

10.1099/00221287-139-10-2371

PMID

unavailable

Abstract

The attachment site (attP) of phage ∅U, a lysogenic phage of Rhizobium leguminosarum biovar trifolii, was identified on a 6. 0 kb EcoRI fragment of the phage DNA. Plasmid pC16 was constructed by cloning this EcoRI fragment into the EcoRI site of suicide plasmid vector pSUP202. Escherichia coli S17-1 harbouring plasmid pC16 was mated with wild-type R. leguminosarum biovar trifolii strain 4S and its lysogenic strain 4S(∅U) using tetracycline resistance (Tc(r)) as a selection marker. The Tc(r) R. leguminosarum biovar trifolii transconjugants appeared at high frequency (10-3-10-4 per recipient cell in both matings). Southern hybridization with the attP fragment and pSUP202 as probes indicated that plasmid pC16 integrated into the chromosome of all these transconjugants in the same manner as phage ∅U.

Language: en

Keywords

suicide; article; priority journal; nonhuman; antibiotic resistance; Escherichia coli; plasmid; bacteriophage; chromosome; DNA fragment; molecular cloning; Rhizobium leguminosarum; Southern blotting