Citation

Turrini F, Vallarino G, Cisani F, Donno D, Beccaro GL, Zunin P, Boggia R, Pittaluga A, Grilli M. Nutrients 2020; 12(11): e3328.

Copyright

(Copyright © 2020, MDPI Publishing)

DOI

10.3390/nu12113328

PMID

33138077

PMCID

PMC7693450

Abstract

Anxiety disorders are common and complex psychiatric syndromes affecting a broad spectrum of patients. On top of that, we know that aging produces an increase in anxiety vulnerability and sedative consumption. Moreover, stress disorders frequently show a clear gender susceptibility. Currently, the approved pharmacological strategies have severe side effects such as hallucinations, addiction, suicide, insomnia, and loss of motor coordination. Dietary integration with supplements represents an intriguing strategy for improving the efficacy and the safety of synthetic anxiolytics. Accordingly, a recent article demonstrated that glyceric bud extracts from Tilia tomentosa Moench (TTBEs) exert effects that are consistent with anxiolytic activity. However, the effects of these compounds in vivo are unknown. To examine this question, we conducted behavioral analysis in mice. A total of 21 days of oral supplements (vehicle and TTBEs) were assessed by Light Dark and Hole Board tests in male and female mice (young, 3 months; old, 24 months). Interestingly, the principal component analysis revealed gender and age-specific behavioral modulations. Moreover, the diet integration with the botanicals did not modify the body weight gain and the daily intake of water. Our results support the use of TTBEs as dietary supplements for anxiolytic purposes and unveil age and gender-dependent responses.

Language: en

Keywords

Animals; Anti-Anxiety Agents; anxiety; Anxiety Disorders; Behavior, Animal; bud-derivatives; Dietary Supplements; Disease Models, Animal; Female; Male; Maze Learning; mice behaviour; mood disorders; nutraceuticals; Plant Extracts; polyphenols; principal component analysis; Principal Component Analysis; Sex Factors; supplements; Tilia