Citation

Sim J, Kim M, Kim S, Yang W. J. Forensic Sci. 2022; 67(1): 353-357.

Copyright

(Copyright © 2022, American Society for Testing and Materials, Publisher John Wiley and Sons)

DOI

10.1111/1556-4029.14896

PMID

34585376

Abstract

Cyanide is a highly toxic agent that has been frequently used for suicide in South Korea. It is also used in various industrial fields, such as metal plating, in which many accidental cyanide intoxications have occurred. To overcome the disadvantages of conventional cyanide analysis methods, a simple and fast method for the analysis of cyanide in whole blood using ion chromatography (IC) with amperometric detection was developed in this study. Whole blood samples were deproteinized, diluted, and analyzed using an IC-amperometric detection system. The limits of detection and quantitation were 0.1 and 0.2 mg/L, respectively. The method showed good linearity in the range of 0.2 to 50 mg/L with R2  > 0.99. The intra- and inter-assay precision and accuracy values were <10%. The established method was successfully applied to analyze whole blood samples from three cyanide intoxication cases.

Language: en

Keywords

Humans; postmortem; Chromatography; forensic toxicology; Republic of Korea; Forensic Toxicology; Cyanides; whole blood; amperometric detection; cyanide intoxication; ion chromatography