Citation

Alese OO, Ngoupaye GT, Rakgantsho C, Mkhize NV, Zulu S, Mabandla MV. Life Sci. 2020; 253: e117692.

Copyright

(Copyright © 2020, Elsevier Publishing)

DOI

10.1016/j.lfs.2020.117692

PMID

32376271

Abstract

BACKGROUND: Depression is the most significant cause of suicide among neuropsychiatric illnesses. Major depression further affects the quality of life in an individual with epilepsy. The treatment of depression in an epileptic patient could be very challenging because of drug selection or the fact that some antiepileptic drugs are known to cause depression. It has been shown that in addition to the known involvement of the serotonergic pathway in depression, the glutamatergic system is also involved in the evolution of the disease, but this knowledge is limited. This study assessed if induction of epilepsy in rats will cause depressive-like behavior, alters the concentrations of metabotropic receptor 5 (mGluR5), glutamate transport protein (GLAST), glutamate synthase (GS) and brain derived neurotrophic factor (BDNF).
MATERIALS AND METHOD: Epilepsy was induced in rats by injecting Pentylenetetrazole at 35 mg/kg every other day. At kindle, rats were subjected to sucrose preference test (SPT) and forced swim test (FST) and decapitated 4 h later. Hippocampal tissue was collected and the BDNF concentration was measured with ELISA; mGluR5 and GS protein expression was measured using western blot while amygdala tissue was used for GLAST expression with flow cytometry.
RESULTS: Our results showed that epilepsy leads to depressive-like behavior in rats and alters the glutamatergic system.
CONCLUSION: Therefore, we conclude that targeting the glutamate pathway may be a good strategy to alleviate depressive-like behavior associated with epilepsy.

Language: en

Keywords

Male; Depression; Animals; Epilepsy; Rats; BDNF; Amygdala; Hippocampus; Brain-Derived Neurotrophic Factor; Rats, Sprague-Dawley; Major depression; Glutamic Acid; Excitatory Amino Acid Transporter 1; Glutamate synthase; Glutamate Synthase; mGluR5; Pentylenetetrazole; Prolonged febrile seizures; Receptor, Metabotropic Glutamate 5; Seizures, Febrile