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Abstract
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OBJECTIVE: To investigate the effects of puerarin on the proliferation, apoptosis and migration of hormone-independent prostate cancer cells, and to explore the possible mechanisms.
METHODS: The effects of puerarin on the proliferation, apoptosis and cell cycle distribution of prostate cancer PC3 cells were detected by CCK-8 and FCM assay, respectively. The effects of puerarin on the migration and invasion of prostate cancer PC3 cells were detected by Transwell chamber assay. The effects of puerarin on the expressions of apoptosis-associated genes and proteins as well as the activity of protein kinase B (PKB, also known as Akt) in prostate cancer PC3 cells were detected by real-time fluorescent quantitative PCR and Western blotting, respectively.
RESULTS: As compared with the control group without intervention, the different concentrations of puerarin could significantly inhibit the proliferation of PC3 cells (all P < 0.05), induce the apoptosis of PC3 cells (all P < 0.01), arrest the PC3 cells at G2/M phase (all P < 0.05), and also effectively inhibit the migration and invasion of PC3 cells (all P < 0.01). The expression levels of phospho-Akt (p-Akt) protein (all P < 0.01) and anti-apoptotic molecule B-cell lymphoma-2 (Bcl-2) mRNA and protein (all P < 0.05) in PC3 cells treated with different concentrations of puerarin were significantly lower than those in the control group. The expression levels of apoptotic molecule Bcl-2-associated X (Bax) mRNA (all P < 0.05) and protein (all P < 0.01), factor associated suicide (Fas) mRNA (all P < 0.01), caspase-3 mRNA (all P < 0.01), cleaved caspase-3 protein (all P < 0.01) and caspase-8 mRNA (all P < 0.01) in PC3 cells treated with different concentrations of puerarin were significantly higher than those in the control group.
CONCLUSION: Puerarin can inhibit the proliferation, migration and invasion of hormone-independent prostate cancer cells, and also promote apoptosis by up-regulating the activity of caspase-3.
Language: zh |