Citation

Xu D. Journal of Chongqing Medical University 2007; (12).

Copyright

(Copyright © 2007)

DOI

unavailable

PMID

unavailable

Abstract

OBJECTIVE:To study combination AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro.

METHODS:The recombinant AdCD-UPRT adenovirus was packaged and generated,and the title of virus was measured. The efficiency of transfecting target cells was detected,the toxicity of virus was measured,and the expression of CD-UPRT gene in A549 cells was assayed. The cytotoxicity and the bystander effect on A549 cells were measured by MTT assays respectively. The apoptosis was analyzed by AO-EB fluorescent staining,transmission electron microscope and flow cytometry.

RESULTS:The combination AdCD-UPRT had obviously growth inhibition effect on A549 cell and bystander effect. The cells were blocked in S phase and induced into apoptosis.

CONCLUSION:The combination AdCD-UPRT suicide gene had cytotoxicity on lung cancer A549 in vitro.

Language: zh

Keywords

Gene therapy; Suicide gene; Adenovirus; Lung neoplasms