Citation

Chang X, Wang Z, Zhang Y, Li Z, Zhao Z, Ma D, Sun J. Chinese J. Gen. Surg. 2001; (12).

Copyright

(Copyright © 2001)

DOI

unavailable

PMID

unavailable

Abstract

OBJECTIVE To investigate cell-killing and bystander effects on gallbladder cancer (GBC-SD) cells transferred with double suicide genes (CD and HSV-tk).

METHODS CD and HSV-tk genes were transfected into PA317 cells using lipofectamine. GBC-SD cells were infected with viral supernatant. GBC-SD/CD+tk cells were treated with 5-FC and/or GCV. GBC-SD/CD+tk and GBC-SD were inoculated subcutaneously into the nude mice respectively and treated with GCV and 5-FC for 21 days.

RESULTS The killing effect of 5-FC and GCV in combination on GBC-SD/CD+tk cells is more effective than using 5-FC or GCV alone. The local bystander effect is significant while the distant bystander effect was not obvious.

CONCLUSION The cell-killing efficacy of chemotherapy on GBC-SD transfected with double suicide gene was significantly increased, and the bystander effect was obvious.

Language: zh

Keywords

Genes; Gallbladder neoplasms; Gene targeting