Citation

Zhou S, Liu C, Gong J, Liu Z, Tang Y, Li S, Xu Y, Ai Z. Zhonghua Gan Zang Bing Za Zhi 2010; (12): 276-279.

Copyright

(Copyright © 2010, Chongqing yi ke da xue di er lin chuang xue yuan bing du xing gan yan yan jiu suo)

DOI

10.3760/cma.j.issn.1007-3418.2010.04.010

PMID

unavailable

Abstract

OBJECTIVETo observe the effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase hepatocellular carcinoma in mice.

METHODSKunming mice were inoculated subcutaneously with H22 tumor cells. 40 male mice bearing subcutaneous hepatoma were randomized into 4 groups: PBS (group A), HSV1-TK (group B), HSV1-TK (group C), and microbubble carrying HSV1-TK (group D) were injected into the tail vein every 3 days. Mice in group C and D were exposed to ultrasound. The expression of TK protein was detected by western blot. Ganciclovir (GCV) was intraperitoneally injected at a dose of 100 mg x kg (-1) x d(-1) in group B, group C and group D. The tumor size was measured every 2 days.

RESULTSTK gene could be injected precisely into hepatocellular carcinoma with ultrasound monitor, and the expression of TK protein was found in all 4 groups. Expression in group D was higher than others (P < 0.05). The rate of tumor growth inhibition were 0 in group A, 3.90%+/-1.80% in group B, 22.70%+/-2.86% in group C, 41.25%+/-3.20% in group D (group B vs group C, P < 0.05; group D vs group C, P < 0.05; group D vs group B, P < 0.05).

CONCLUSIONUltrasound microbubble not only improve target gene therapy, but also enhance transfection efficiency.

Language: zh