Citation

Li H, Xiang S, Ma N, Hu W, Zeng Z. Zhong Nan Da Xue Xue Bao Yi Xue Ban 2011; (12): 836-843.

Copyright

(Copyright © 2011, Zhong Nan Da Xue Xue Bao)

DOI

10.3969/j.issn.1672-7347.2011.09.004

PMID

unavailable

Abstract

OBJECTIVE@#To determine the effect and molecular mechanism of DNA damage caused by suicide gene therapy system HSV-TK/GCV under Tet-On regulation in human breast cancer cell line MCF-7 infected by recombinant adeno-associated virus (rAAV).@*METHODS@#We used comet assay to detect the effect of HSV-TK/GCV suicide gene regulation system on MCF-7 DNA damage, and analyzed the expression change of relative DNA damage response active genes and proteins with RT-PCR and Western blot.@*RESULTS@#Compared with other control groups, the comet assay showed that MCF-7 cells with HSV-TK/GCV treatment had obvious comet tails, and the expression level of DNA damage response active genes and proteins changed obviously in the HSV-TK/GCV treatment group,such as ATM, p53 and p27,but CyclinE and CDK2 did not change.@*CONCLUSION@#DNA damage on MCF-7 cells is resulted from HSV-TK/GCV in suicide gene therapy system through a p53-dependent signal pathway, causing cell cycle arrest and cell death.

Language: zh