Citation

Qing J, Zhao S, Jiang W, Zhang H. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2014; (24): 178-181.

Copyright

(Copyright © 2014, Lin chuang er bi yan hou tou jing wai ke za zhi bain ji bu)

DOI

unavailable

PMID

unavailable

Abstract

OBJECTIVE@#To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49.@*METHOD@#The plasmids of pcDNA3.1 (-)E6. BARF1p. CD/UPRT. UL49 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UL49 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE-2 cells.@*RESULT@#Suicide genes were expressed stably in CNE-2 cells.@*CONCLUSION@#We constructed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamine.

Language: zh