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Citation
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Chen S, Li J, Yu C, He L, Zhang C, Cheng X, Li Y, Yan Y, Jin X, Chen G, Zhao Z. Chinese Journal of Immunology 2015; (12): 215-220. |
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Abstract
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OBJECTIVE:In order to develop a safer vaccine strain exploit Salmonella typhimurium vaccine strain.A ΔsipB mutant of Salmonella typhimurium SL 1344 strain was constructed.
METHODS: Firstly, the recombinant suicide plasmid containing the missing 585 bp sipB ( PREΔsipB ) was built by homologous recombination , and screenned by two-step method.
RESULTS: PCR and sequencing results showed that SL 1344ΔsipB was successfully constructed.It was no significant changes compared with SL 1344.But compared with the parent strains SL 1344 , the mutant strain had obvious change in its virulence , oral challenge of bacteria in mice revealed that LD50 of the mutant strain was 1.70 ×108 CFU,the toxicity reduced about 1.4%.The protection rate induced by the sipB mutant was 50%,and the serum antibody peaked 14 d post-immunization.
CONCLUSION:The SL1344ΔsipB mutant was constructed suc-cessfully,and genetic stability ,significantly reduced virulence.The study provides a new approach for further study of the relationship between the gene and pathogenicity of Salmonella typhimurium.It is likely that the ΔsipB mutant could be adapted to develope attenuated Salmonella vaccine.
Language: zh |