Citation

Guo L, Liu J, Li J, Et A. Chinese Journal of Immunology 2000; (12).

Copyright

(Copyright © 2000)

DOI

unavailable

PMID

unavailable

Abstract

OBJECTIVE:It was aimed to highlight the pro apoptotic significance and immunotherapeutic value of the membrane type Fas(mFas)up regulated in all trans retinoic acid(RA)differentiated medulloblastoma cells Med 3.

METHODS:Med 3 cells were treated by 10 ?mol/L RA for 0,24,48 or 72 h,followed by the supplementation of anti Fas antibody in the final concentration of 25,50,100,200 or 400 ng/ml.The cell morphology ,growth and death pattern(s)were evaluated by multiple approaches.

RESULTS:RA could promote cell differentiation and mFas expression but not induce apoptosis.Anti Fas antibody alone had no biological effect on Med 3 cells but when combined with 10 ?mol/L RA,showed pro apoptotic activity in a dose related fashion.The best apoptotic induction could be achieved in the cell population pretreated by RA for 48 h.

CONCLUSION:The RA enhanced apoptotic susceptibility of Med 3 cells is closely associated with mFas up regulation.The chemical activation of Fas suicide system may be a novel regimen for medulloblastomas.

Language: zh

Keywords

All trans retinoic acid Medulloblastoma Differentiation Anti Fas antibody Apoptosi