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Abstract
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OBJECTIVE: To investigate the therapeutic effect of 125 I seeds interstitial brachytherapy on the subcutaneously implanted hepatocellular carcinoma in mice.
METHODS: H 22 cells (2 × 107/0.2 mL) were transplanted subcutaneously in the right lateral back of 22 BALB/c nude mice. The xenografted tumor model was established after 15 d. The mean diameter of tumor was 12 mm. The tumor-beared nude mice were divided into a 125 I seeds interstitial brachytherapy group (n = 12) and a control group (n = 10). The survival rates of mice and the tumor size were measured. After 25 d, nude mice were executed and pathological examination was performed. The expression of the proliferation cell nuclear antigen (PCNA) and factor-associated suicide (FAS) were determined by immunohistochemical methods. The ratio of microvascular density (MVD) in the two groups was calculated.
RESULTS: At d 25 after seeds implantation, the survival rates were 83.3% and 50% and the mean tumor volumes were (381.5 ± 72.64) mm3 and (5 620.9 ± 969.8) mm3 in the treatment group and control group, respectively. The difference was significant between the two groups (P < 0.01). Histological analysis revealed coagulative necrosis, inflammatory cell invasion and fibrosis in the surrounding area of the implantation site of radioactive seeds, whereas in the surrounding area of the dummy seeds only slight fibrosis and edema were observed, with proliferation of tumor cells. Immunohistochemistry analysis showed that there was apparent apoptosis of tumor cells in 125 I seeds implantation group. There was significant difference between the treatment group and the control group.
CONCLUSION: 125 I seed interstitial brachytherapy inhibites the growth of tumor by directly killing tumor cells, inhibiting its proliferation and inducing apoptosis.
Language: zh |