Citation

Wang J, Hao R, Jiang T, Guo X, Zhou F, Cao L, Gao F, Wang G, Wang J, Ning K, Zhong C, Chen X, Huang Y, Xu J, Gao S. Cell and Bioscience 2022; 12(1).

Copyright

(Copyright © 2022)

DOI

10.1186/s13578-022-00774-x

PMID

unavailable

Abstract

BACKGROUND: Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding.

RESULTS: The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15.

CONCLUSION: Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. © 2022, The Author(s).

Language: en

Keywords

memory; human; hippocampus; controlled study; human cell; apoptosis; cell proliferation; nonhuman; animal cell; mouse; cell differentiation; animal experiment; animal model; interleukin 2; Article; animal tissue; brain cortex; hypothalamus; interleukin 1beta; in vivo study; immunomodulation; cell survival; corpus striatum; glial fibrillary acidic protein; long term potentiation; ganciclovir; interleukin 15; Ki 67 antigen; middle cerebral artery occlusion; excitatory postsynaptic potential; gamma interferon inducible protein 10; ischemic stroke; action potential; adipose derived stem cell; Adipose-derived stem cells (ADSCs); cell migration; hADSC-derived neuron-like cells (hADSC-NCs); interleukin 1alpha; macrophage inflammatory protein 1beta; microtubule associated protein 2; Middle cerebral artery occlusion (MCAO); monocyte chemotactic protein 1; National Institute of Health Stroke Scale (NIHSS); Rogers scale system; spatial learning