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Citation |
Hristova SH, Zhivkov AM. Comptes Rendus de L'Academie Bulgare des Sciences 2019; 72(2): 198-203. |
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Copyright |
(Copyright © 2019) |
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DOI |
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PMID |
unavailable |
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Abstract |
Cytochrome c (cytC) is electron-transporting haemoproteid associated with the inner mitochondrial membrane, which plays an important role in the process of apoptosis (a suicide of functionally non-effective cells). In the case of cancer cells the apoptosis is not working but the process can be unblocked by input of exogenous cytC in the cytoplasm. Our experiments with cancer cell culture show out that cytC has cytotoxic effect when it is adsorbed on montmorillonite (MM, negatively charged plate-like colloid particles), but there is no effect when the protein and the particles are added separately. These facts reflect: (a) the ability of cancer cells to phagocytize particles with submicron size, (b) the low cytotoxicity of MM plates, and (c) the impermeability of the cytoplasmic membrane for cytC globules. In the present work we report results obtained by the methods of protein electrostatics and microelectrophoresis, which allow reaching maximal cytotoxic effect at 0.5 µm MM diameter, saturated cytC adsorption, positively recharged cytC-MM particles, w/w 10:3 ratio of cytC to MM. The test on metastatic colon cancer line shows that the cytotoxicity reaches 94% at 96th hour after addition of the cytC-MM suspension to the cell culture. The studying of the literature discloses that this result is significantly better than the reached by other authors using cytC adsorbed on different colloid particles and different cell lines. © 2019, Academic Publishing House. All rights reserved. Language: en |
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Keywords |
Cytochrome c; Cancer cell culture; Colloid particles; Cytotoxicity; Microelectrophoresis; Montmorillonite; Protein adsorption; Protein electrostatics |