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Citation |
Li X, Shi J, Chen C, Li W, Han L, Lan L, Guo Y, Chang Y, Cai J, Ding Y. New Journal of Chemistry 2018; 42(8): 6293-6298. |
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Copyright |
(Copyright © 2018) |
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DOI |
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PMID |
unavailable |
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Abstract |
The ingestion of organophosphate (Op) pesticides for attempting suicide has been a serious health issue recently. Phorate, a class of Op, has attracted attention in this regard and so the rapid diagnosis and detection of phorate is crucial for human health. In this study, a one-step and visual method for the rapid determination of phorate via an aptasensor based on DNA-templated silver nanoclusters (DNA-AgNCs) has been presented. The designed DNA-AgNC nanoprobe, containing a special structure with an intercalated aptamer, shows excellent stability. Introducing phorate to the system easily triggers the aggregation of DNA-AgNCs, producing a brown-to-colorless color change in the solution in only 6 minutes. Under the optimal conditions, the aptasensor can allow for the determination of phorate in the concentration range of 0-25 μg mL-1, using two linear equations: y = -2.308x + 0.996 (R2 = 0.977) for 0-0.125 μg mL-1 and y = -0.020x + 0.664 (R2 = 0.971) for 0.125-25 μg mL-1. The estimated detection limit of phorate was 0.012 ng mL-1. Moreover, the practicability of this proposed method was also validated by analyzing phorate-spiked human blood samples and the results were in agreement with those from gas chromatography/mass spectrometry (GC/MS) analysis. Therefore, this fabricated aptasensor is suitable for sensing phorate in complex biological samples, and shows high potential for clinical diagnosis or forensic toxicology analysis. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique. Language: en |
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Keywords |
human; DNA; forensic toxicology; controlled study; blood sampling; priority journal; sensitivity and specificity; concentration (parameters); phorate; blood analysis; quantitative analysis; mass fragmentography; transmission electron microscopy; Article; buffer; fluorescence; silver; limit of detection; aptamer |