Citation

Reject Paul PM, Singh RP. International Journal of ChemTech Research 2015; 8(6): 804-817.

Copyright

(Copyright © 2015)

DOI

unavailable

PMID

unavailable

Abstract

In this study a working electrode was constructed by immobilization of Acetyl choline esterase, on gold nanoparticles synthesized by electrochemical deposition method on synthetic graphite, using cross linker, gluteraldehyde and 2-Aminoethane thiol for the determination of monocrotophos, an organophosphorus pesticide in blood serum by inhibition of AChE using Cyclic voltammetry. The monocrotophos concentration response was linear in the range from 0.05mgL-1 to 3.2mg L-1.The detection limit for Monocrotophos was 0.05mg L-1. The results of the present study based on synthetic graphite which being a better conducting electrode offers as a good alternative to other conventional methods as it is economical and with better sensitivity for the detection of analytes like organophosphorus compounds from body fluids for the forensic science related works as the other methods like Gas chromatography/mass spectrometry are costly and time consuming.. The resultant modified graphite electrode surface was characterized using Scanning electron microscopy, EDX and AFM. Immobilization of AChE on AuNPs/Graphite composite is a new approach. The electrodes based on graphite and AuNPs being economical, good conductor offers as a better option for the sensitive electrochemical detection of organophosphorus compounds from body fluids in forensic science scenario in cases of murder, suicide, accidental poisoning, and chemical warfare. © 2015, Sphinx Knowledge House. All rights reserved.

Language: en

Keywords

acetylcholinesterase; serum; oxidation; pH; forensic science; electrode; enzyme immobilization; mass fragmentography; Article; Immobilization; temperature; monocrotophos; electrochemical detection; body fluid; scanning electron microscopy; Organophosphorus pesticide; electric potential; process optimization; Acetylcholine esterase; atomic force microscopy; Forensic Science; gold nanoparticle; Gold nanoparticles; graphite; potentiometry; Synthetic graphite; thiocholine