Citation

Sridevi A, Narasimha G, Sandhya A, Devi PS. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2015; 6(4): 1042-1049.

Copyright

(Copyright © 2015)

DOI

unavailable

PMID

unavailable

Abstract

Production of cellulase components, exoglucanase, endoglucanase and β-glucosidase by a local fungal isolate of A. niger in submerged fermentation on cellulose and lignocelluloses was studied. The effect of varying optimal cultural and nutritional parameters - incubation period, substrate concentration, pH, and temperature were evaluated for optimal production of enzymes. The growth of the fungus on the medium with 1% cellulose concentration, pH 5.0 at 30°C after 5-day incubation yielded higher titers of cellulase. Control of pH during the course of the growth of the fungus enhanced enzyme production. Supplementation of culture medium with natural lignocellulose induced higher production of cellulase than with cellulose substrate.

Language: en

Keywords

controlled study; nonhuman; enzyme activity; pH; suicide substrate; Article; incubation time; enzyme assay; Fungi; enzyme synthesis; incubation temperature; cellulose; culture medium; A.niger; Aspergillus niger; beta glucosidase; cellulase; Cellulase; fermentation medium; fermentation optimization; fungus culture; fungus growth; glucan glucosidase; glucan synthase; lignocellulose; Optimal conditions