Citation

Jiao LF, Yan CH, Zhao J, Jia L, He J, Peng SQ. Chinese Journal of Pharmacology and Toxicology 2011; 25(6): 568-575.

Copyright

(Copyright © 2011)

DOI

10.3867/j.issn.1000-3002.2011.06.009

PMID

unavailable

Abstract

OBJECTIVE: To investigate effect of chlorpyrifos on reproductive toxicity in male rats and explore the possible mechanisms.

METHODS: Male Wistar rats were ig given CPF 1, 5 and 10 mg·kg -1 or olive oil vehicle (normal control group) daily for 12 consecutive weeks. Testicular and epididymal viscera weight and organ coefficients were calculated, testicular histopathologic changes were evaluated by hematoxylin and eosin (HE) staining, the sperm abnormality, total sperm count and the sperm mobility were examined. The activities of alkaline phosphatase (AKP), acid phosphatase(ACP), lactate dehydrogenase(LDH) and γ-glutamyltranspeptidase (γ-GT) were measured. The germ cell apoptosis of testicle was evaluated with TUNEL assay. The expression of factor associated suicide/factor associated suicide ligand (Fas/FasL) and caspase-3 was analyzed by immunohistochemistry and Western blot.

RESULTS: Compared with normal control group, CPF induced significant increase in the testicular coefficients(P < 0.05) and tendency increases in epididymal viscera. The total sperm counts and the sperm mobility percentage decreased and the sperm abnormality increased in CPF groups(P < 0.05). There was significant decrease in AKP and γ-GT activities (P < 0.05) in CPF groups. The histopathological examination showed that CPF could lead to atrophia of the contorted seminiferous tubules, and decrease of spermatogenic cell. The TUNEL results showed the significant apoptosis of spermatogenic cells (P < 0.05). The expression of Fas/FasL and caspase-3 in testis was up-regulated.

CONCLUSION: Repeated exposures to low dose of CPF may induce the male reproductive toxicity, the Fas/FasL regulated germ cell apoptosis may be one of the mechanism.

Language: zh

Keywords

male; chlorpyrifos; Apoptosis; immunohistochemistry; article; controlled study; apoptosis; nonhuman; animal cell; enzyme activity; animal experiment; animal model; rat; reproductive toxicity; protein expression; histopathology; Mechanism; lactate dehydrogenase; gamma glutamyltransferase; olive oil; Chlorpyrifos; Western blotting; caspase 3; nick end labeling; Rattus; testis atrophy; enzyme induction; alkaline phosphatase; testis weight; staining; Wistar rat; acid phosphatase; eosin; Fas/FasL; germ cell; hematoxylin; Rattus norvegicus; Reproductive toxicity; semen abnormality; seminiferous tubule; spermatocyte; spermatozoon count; spermatozoon motility