Citation

Fujlta Y, Tokunaga I, Gotohda T, Ishigami A, Kubo SI. Acta Criminol. Med. Leg. Jpn. 2003; 69(3): 75-79.

Copyright

(Copyright © 2003, Japanese Association of Criminology)

DOI

unavailable

PMID

unavailable

Abstract

When an unidentified body is found, DNA typing offers an important information for identification. In most cases, however, DNA source materials are not available for missingindividuals to be compared with the body sample. So the DNA typing of the family membersof the suspected individual is requested. Three skeletal remains were found in the sea: anadult woman, an elder child, and a younger child. They were suspected to be a missing mother and her two daughters whose father had been killed the year before. The father's blood had been stored at post-mortem and was available for the identification test of this family. The woman's skeleton was judged to be the mother from the feature of the dental chart. DNA's were extracted from the thigh bones of the three remains and submitted for identification. The MCT118 system failed to be typed in all three bodies. Other DNA polymorphic systems: HLA DQA1, TH01 and PM (LDLR, GYPA, HBGG, D7S8, GC), were successfully testedfor the mother and the elder child but could not be typed at all for the younger child. The cause of the typing failure was examined. Effect of PCR-inhibiting materials was excluded by correct typing results of known DNA samples added to the sample of the younger child. Therefore, the DNA of the younger child was estimated to have been decomposed into smaller fragments of about 100 bp. he three bodies were found in the vehicle half buried in the mud of the sea bottom. The younger child's body was buried deeper in the mud and the bones were stained darker than those of the mother and the elder child. This situationsuggested the cause of the younger child's DNA to be highlier fragmented. Finally, the parent-child relationship was analyzed by comparing the ABO and the DNA types of the familysamples. The paternity of the father was confirmed by the paternity likelihood of 99.61%.

Language: ja

Keywords

PCR; DNA type; Personal identification; Postmortem changes; Skeletal remain