Citation

Balandin AA, Balandin VA, Balandina IA, Zheleznov LM. Journal of Global Pharma Technology 2017; 9(3): 42-48.

Copyright

(Copyright © 2017)

DOI

unavailable

PMID

unavailable

Abstract

The objective of the study is to establish regularities of age changes in the structural organization of the cerebellar cortex in old age as compared to young age by using necropsy material. Craniometrical, organometric, histological, immunomorphological and morphometric studies of the cerebellar cortex in 219 human corpses (108 men and 111 women) aged from 18 to 44 years and from 75 to 90 years. Comparative analysis of the parameters of Purkinje's cells in these age groups revealed a decrease in the neurons' height and width. It was found that the distance between the bodies of the neurons of ganglionic layer increases with age, which is obviously due to the progressing disorganization and apoptotic death of Purkinje's cells. An increase in the number of astrocytes immunopositive to glial fibrillary acidic protein, S-100 protein and vimentin in the granular and molecular layers of the cerebellar cortex is observed, as well as a decrease in the number of Purkinje's cells immunopositive to neuron-specific enolase and immunonegative to S-100 protein and vimentin, which can be considered as a manifestation of neurodegeneration. Immunohistochemical techniques provide a more differentiated approach to the morphological assessment of the cerebellar cortex in old persons and make it possible to get more objective and complete information about the postnatal morphogenesis. © 2009-2017, JGPT. All Rights Reserved.

Language: en

Keywords

Glia; Glial fibrillary acidic protein; Men; Neuron-specific Enolase; Old age; S-100 Protein; Vimentin; Women; Youth