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Journal Article

Citation

Du YK, Zhang TY, Liu JJ, Liu C, Kang XD, Zheng DY, Shi H, Xu QY, Ma KJ, Zhao J. Fa Yi Xue Za Zhi 2022; 38(1): 110-113.

Copyright

(Copyright © 2022, Si fa bu Si fa jian ding ke xue ji shu yan jiu suo)

DOI

10.12116/j.issn.1004-5619.2021.410707

PMID

35725713

Abstract

OBJECTIVES: To retrospectively analyze diatom test cases of corpses in water and discuss the value of quantitative analysis of diatoms in the diagnosis of drowning.

METHODS: A total of 490 cases of water-related death were collected. They were divided into drowning group and postmortem immersion group according to the cause of death. Diatoms in lung, liver, kidney tissue and water sample were analyzed quantitatively by microwave digestion-vacuum filtration-automated scanning electron microscopy (MD-VF-Auto SEM) method. The ratios of content of diatoms in lung tissue and water sample (C(L)/C(D)) were calculated.

RESULTS: The results of diatom test for three organs (lung, liver and kidney) were all positive in 400 cases (85.5%); the content of diatom in lung, liver, kidney tissues, and water samples of drowning group were (113 235.9±317 868.1), (26.7±75.6), (23.3±52.2) and (12 113.3±21 760.0) cells/10 g, respectively; the species of diatom were (7.5±2.8), (2.6±1.9), (2.9±2.1) and (8.9±3.0) types, respectively; the C(L)/C(D) of drowning group and postmortem immersion group were (100.6±830.7) and (0.3±0.4), respectively.

CONCLUSIONS: Quantitative analysis of diatoms can provide supportive evidence for the diagnosis of drowning, and the parameter C(L)/C(D) can be introduced into the analysis to make a more accurate diagnosis of drowning.


Language: zh

Keywords

drowning; forensic pathology; diatom test; microwave digestion-vacuum filtration-automated scanning electron microscopy method; quantitative analysis

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