Citation

Yesildag K, Kokulu K, Mutlu H, Eroz R, Taha-Sert E, Saritas A. Gac. Med. Mex. 2021; 157(6): 610-617.

Copyright

(Copyright © 2021, Academia Nacional De Medicina de Mexico)

DOI

10.24875/GMM.M21000625

PMID

35108251

Abstract

OBJECTIVES: The purpose of the study is to investigate whether there is any relationship between mean argyrophilic nucleolar organizing regions (AgNOR) number and total AgNOR area/total nuclear area (TAA/TNA) ratio and the levels of brain hypoxia after exposure to different acute doses of carbon monoxide (CO) gas.

METHODS: Each experimental group was exposed to CO gas (concentrations of 1,000, 3,000 and 5,000 ppm). Then, the rats were anesthetized, and blood samples were taken from the right jugular vein for carboxyhemoglobin levels detection. The rats were sacrificed on seventh day. AgNOR staining was applied to brain tissues. TAA/TNA and mean AgNOR number were detected for each nucleus.

RESULTS: Significant differences were detected among all groups for TAA/TNA ratio, mean AgNOR number and carboxyhemoglobin level. According to a double comparison of groups, the differences between control and 1,000 ppm, control and 3,000 ppm, control and 5,000 ppm, and between 1,000 and 5,000 ppm were significant for TAA/TNA ratio. When mean AgNOR number was considered, significant differences were detected between control and 1,000 ppm, control and 3,000 ppm, control and 5,000 ppm, and between 1,000 and 3,000 ppm.

CONCLUSION: AgNOR proteins may be used for early detection of the duration, intensity, and damage of brain injury caused by CO poisoning. Thus, effective treatment strategies can be developed for the prevention of hypoxic conditions.

Language: en

Keywords

Brain; ADNr; Argyrophilic nucleolar organizer regions; Cerebro; CO poisoning; Intoxicación por CO; Nucleolar organizer regions; rDNA; Regiones organizadoras nucleolares; Regiones organizadoras nucleolares argirofílicas