Citation

Moore-Lotridge SN, Ihejirika R, Gibson BHY, Posey SL, Mignemi NA, Cole HA, Hawley GD, Uppuganti S, Nyman JS, Schoenecker JG. Bone Rep. 2021; 14: e100743.

Copyright

(Copyright © 2021, Elsevier Publishing)

DOI

10.1016/j.bonr.2020.100743

PMID

33490313

Abstract

Severely injured patients are beleaguered by complications during convalescence, such as dysregulated biomineralization. Paradoxically, severely injured patients experience the loss of bone (osteoporosis), resulting in diminished skeletal integrity and increased risk of fragility fractures; yet they also accrue mineralization in soft tissues, resulting in complications such as heterotopic ossification (HO). The pathophysiology leading to dysregulated biomineralization in severely injured patients is not well defined. It has been postulated that these pathologies are linked, such that mineralization is "transferred" from the bone to soft tissue compartments. The goal of this study was to determine if severe injury-induced osteoporosis and soft tissue calcification are temporally coincident following injury. Using a murine model of combined burn and skeletal muscle injury to model severe injury, it was determined that mice developed significant progressive bone loss, detectable as early as 3 days post injury, and marked soft tissue mineralization by 7 days after injury. The observed temporal concordance between the development of severe injury-induced osteoporosis and soft tissue mineralization indicates the plausibility that these complications share a common pathophysiology, though further experiments are required.

Language: en

Keywords

Trauma; Severe injury; Burn; Biomineralization; BMD, bone mineral density; BV/TV, bone volume/tissue volume; CTX, cardiotoxin; DC, dystrophic calcification; DPI, days post injury; DXA, dual energy X-ray absorptiometry; Dystrophic calcification; H&E, hematoxylin and eosin; Heterotopic ossificaiton; HO, heterotopic ossification; Osteoporosis; Severe injury-induced osteoporosis; Soft tissue mineralization; STiCSS, soft tissue calcification scoring system; μCT, microcomputed tomography