Citation

Plouffe BD, Murthy SK. Electrophoresis 2017; 38(3-4): 501-506.

Affiliation

Department of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, USA.

Copyright

(Copyright © 2017, Wiley-VCH)

DOI

10.1002/elps.201600075

PMID

27862053

Abstract

With the recent worldwide changes in the legalization of marijuana, there is a significant need for rapid, roadside screening test for driving under the influence of drugs. A robust, sensitive, lateral flow assay has been developed to detect recent use via oral-fluid testing for Δ⁹ -tetrahydrocannabinol (THC). This proof-of-concept assay uses a fluorescent-based immunoassay detection of polymeric beads, conjugated to antibodies against native THC. The fluorescent technique allows for significantly lower limits of detection and higher precision determination of recent marijuana use without the use of urine or blood sampling-thus allowing for roadside identification. Detection levels of 0.01 ng/mL were distinguished from background and the lower limit of quantification was determined to approach 1 ng/mL.

Keywords: Cannabis impaired driving


© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Language: en

Keywords

Fluorescence; Lateral flow assay; Marijuana; Roadside detection; THC