Citation

Brunelle E, Thibodeau B, Shoemaker A, Halámek J. ACS Sens. 2019; 4(12): 3318-3324.

Affiliation

Dept. of Chemistry , University at Albany State University of New York , 1400 Washington Ave. , Albany , New York 12222 , United States.

Copyright

(Copyright © 2019, American Chemical Society)

DOI

10.1021/acssensors.9b02020

PMID

31793770

Abstract

The sudden increase in states legalizing marijuana has forced law enforcement into a situation where the use and consumption are legal, but there are no limitations for what is acceptable for driving or operating machinery. Using ultraviolet-visible (UV-vis) spectroscopy, fingerprints from volunteers who had used marijuana were analyzed via a competitive immunoassay for the detection of Δ⁹-tetrahydrocannabinol (Δ⁹-THC), the main psychoactive component of marijuana, and 11-nor-9-carboxy-THC (THC-COOH), one of the main metabolites produced in the body following the use/consumption of THC-related products. In this research, the THC-COOH metabolite and the enzyme-labeled conjugate compete against each other as the antigens for the system. The antibody used in this assay has a greater affinity for the metabolite; so, as its concentration increases, the absorbance of the system decreases due to less binding of the enzyme-labeled conjugate.


Keyword: Cannabis impaired driving

Language: en

Keywords

THC; drug detection; fingerprint; immunoassay; marijuana; metabolite; noninvasive; sweat