Citation

Kakehashi H, Shima N, Ishikawa A, Nitta A, Asai R, Wada M, Nakano S, Matsuta S, Sasaki K, Kamata H, Kamata T, Nishioka H, Miki A, Katagi M. Forensic Sci. Int. 2019; ePub(ePub): ePub.

Affiliation

Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 1-3-18 Hommachi, Chuo-ku, Osaka 541-0053, Japan.

Copyright

(Copyright © 2019, Elsevier Publishing)

DOI

10.1016/j.forsciint.2019.110106

PMID

31902661

Abstract

The influence of lipophilicity and functional groups of synthetic cannabinoids (SCs) on their blood concentrations and urinary excretion has been studied by analyzing blood and urine specimens sampled from drivers who were involved in a car crashes under the influence of SCs. A total of 58 specimens (26 urine and 31 blood specimens), sampled within 13h of the occurrence, were analyzed by liquid chromatography-tandem mass spectrometry. Fifteen SCs were detected in those specimens; the SCs detected were categorized as follows: Class 1, Naphthoyl/Benzoyl indole (EAM2201 and three other analogs); Class 2, Indole-3-carboxylate/carboxamide containing naphthol/quinol (5F-PB-22 and four other analogs); and Class 3, Indazole-3-carboxamide containing valine/tert-leucine derivative (5F-AMB and five other analogs). The calculated lipophilicity index log P, the octanol/water participation coefficient, of those SCs in Classes 1, 2, and 3 ranged between 5.01-8.14, 5.80-6.74 and 2.29-3.81, respectively. Class 3 SCs were detectable in 12 out of 13 urine specimens, but those in Classes 1 and 2 were not detected in urine. Our analytical results indicated that the boundary line for their detectability in urine lies between log P 4 and 5. The blood concentrations of Class 3 SCs varied widely (0.0036-31ng/ml) depending on their log P, while much smaller variation was observed among those in Class 2 (0.10-5.0ng/ml).

Copyright © 2019 Elsevier B.V. All rights reserved.

Language: en

Keywords

Blood; Driving; LC–MS/MS; Pharmacokinetics; Synthetic cannabinoids; Urine