Citation

Wang Q, Li C, Li W, Li Z, Zhang Y, Chen G, Liu H. Angew Chem. Int. Ed. Engl. 2019; ePub(ePub): ePub.

Affiliation

CHINA.

Copyright

(Copyright © 2019, Wiley-VCH)

DOI

10.1002/anie.201911803

PMID

31626380

Abstract

Traumatic brain injury (TBI) is one of the most dangerous acute diseases resulting in high morbidity and mortality. Current methods remain limited with respect to early diagnosis and real-time feedback on the pathological process. Herein, a targeted activatable fluorescent nanoprobe (V&A@Ag 2 S) in the second near-infrared window (NIR-II) is presented for  in vivo  optical imaging of TBI. Initially, the fluorescence of V&A@Ag 2 S displays an "off" state owing to energy transfer from Ag 2 S to the A1094 chromophore. Upon intravenous injection, V&A@Ag 2 S quickly accumulates in the inflamed vascular endothelium of TBI based on VCAM1-mediated endocytosis, after which the nanoprobe achieves rapid recovery of the NIR-II fluorescence of Ag 2 S quantum dots (QDs) due to the bleaching of A1094 by the prodromal biomarker of TBI, peroxynitrite (ONOO -  ). Taking advantage of the deep tissue penetration and high signal-to-noise ratio of NIR-II fluorescence imaging, this nanoprobe offers high specificity, rapid response, and high sensitivity toward ONOO - , providing a convenient approach for  in vivo  early real-time assessment of TBI.

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Language: en

Keywords

early diagnosis; in vivo fluorescence imaging; peroxynitrite; second near-infrared window; traumatic brain injury