Citation

Li Y, Zhao Q, Liu B, Dixon A, Cancio L, Dubick M, Dalle Lucca J. Trauma Surg. Acute Care Open 2019; 4(1): e000217.

Affiliation

Viral Therapeutics, Chemical and Biological Technologies, Fort Belvoir, Virginia, USA.

Copyright

(Copyright © 2019, The author(s) and the American Association for the Surgery of Trauma, Publisher BMJ Publishing Group)

DOI

10.1136/tsaco-2018-000217

PMID

31058236

PMCID

PMC6461142

Abstract

BACKGROUND: Complementopathy (rapid complement activation and consumption after trauma) has been reported in trauma patients, but the underlying mechanism of these phenomena and their clinical significance remain unclear. This study aimed to determine the complement/complement pathway activation and identify the association of complement activation with clinical outcomes in trauma patients.

METHODS: We studied 33 trauma patients with mean Injury Severity Score of 25, and 25 healthy volunteers. Sera were collected on patients' arrival at the emergency department, as well as 1, 2, 3, 5, and 7 days after trauma, to measure the levels of terminal complement activation product soluble C5b-9 (sC5b-9) by ELISA. In addition, the functional complement activation pathway was evaluated using a commercial complement system screening kit.

RESULTS: Serum concentrations of sC5b-9 (complement terminal pathway activity) were significantly increased in trauma patients throughout the entire observation period except on day 1. Complement terminal activities were significantly higher in 27 of 33 patients with systemic inflammatory response syndrome (SIRS) than non-SIRS patients on day 2, day 5, and day 7. Increased serum levels of sC5b-9 positively correlated with SIRS. Functional complement analysis revealed that the classical pathway was the predominant pathway responsible for complement activation. Burn patients tended to have a greater and prolonged classical pathway activation than non-burn patients, and burn injury and blunt injury were associated with higher blood levels of sC5b-9 than penetrating injury.

DISCUSSION: Early complement activation through the classical pathway after trauma is observed and positively correlated with the development of SIRS. Thus, monitoring of the complement system might be beneficial in the care of critically injured patients. LEVEL OF EVIDENCE: III. STUDY TYPE: Prognostic.

Language: en

Keywords

complement system; prognosis; systemic inflammatory response syndrome; trauma