Citation

Chai L, Yang X, Liu M, Liu C, Han L, Guo H, Li C, Sun Y, Li X, Xiao M, Fang Z. Biosci. Rep. 2018; 38(5): ePub.

Affiliation

Taihe Hospital, Shiyan, California, China.

Copyright

(Copyright © 2018, Biochemical Society, Publisher Portland Press)

DOI

10.1042/BSR20181113

PMID

30249752

Abstract

OBJECTIVE: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of this study was to systematically study the epitopes of wasp venom.

METHODS: Using a random 12-peptide phage library, we performed antibody-binding epitope panning on 10 serum samples from wasp sting victims at 3 h and 4 days after the sting. The panning epitopes were identified by high-throughput sequencing and matched with wasp venom proteins by BLAST. The panned antibody-binding epitopes were verified by ELISA.

RESULTS: A total of 35 specific potential wasp venom epitopes in 4 days were identified. Among them, 12 peptide epitopes were matched with nine wasp venom proteins, namely, vitellogenin precursor, hexamerin 70b precursor, venom carboxylesterase-6 precursor, MRJP5, major royal jelly protein 8 precursor, venom acid phosphatase Acph-1 precursor, phospholipase A2, venom serine protease 34 precursor, and major royal jelly protein 9 precursor. The changes in serum IgM antibodies induced by wasp venom were confirmed by ELISA based on the 12 peptide epitopes.

CONCLUSION: The nine wasp venom proteins are potential allergens, which should be excluded or modified in the potential biomedical applications of wasp venom.

©2018 The Author(s).

Language: en

Keywords

allergens; peptide; wasp venom