Citation

Puri P, Kumar N, Sharma D, Shukla SK. Med. Leg. J. 2019; 87(1): 32-35.

Affiliation

Amity Institute of Forensic Sciences, Amity University, Noida, India.

Copyright

(Copyright © 2019, Medico-Legal Society, Publisher SAGE Publications)

DOI

10.1177/0025817218789569

PMID

30070156

Abstract

In many cases of sexual assault, traces of semen are left behind on the victim's body, clothes and the area in which the assault has taken place. The positive identification of semen is instrumental in supporting such cases. There are several methods of forensic examination of semen reported in literature, but the presence of blood complicates the identification of semen stains. This paper presents one such case study where the presence of blood makes DNA profiling more challenging as the PCR amplification becomes complicated, and the absolute differential isolation is the only way to get the clear profile using identifiler kits.

Language: en

Keywords

DNA profiling; PCR; Semen stains; differential isolation; forensic examination