Citation

Silva A, Cristofori-Armstrong B, Rash LD, Hodgson WC, Isbister GK. Cell. Mol. Life Sci. 2018; 75(23): 4465-4478.

Affiliation

Clinical Toxicology Research Group, University of Newcastle, Callaghan, NSW, 2308, Australia. geoff.isbister@gmail.com.

Copyright

(Copyright © 2018, Holtzbrinck Springer Nature Publishing Group)

DOI

10.1007/s00018-018-2893-x

PMID

30069700

Abstract

Snake venom α-neurotoxins potently inhibit rodent nicotinic acetylcholine receptors (nAChRs), but their activity on human receptors and their role in human paralysis from snakebite remain unclear. We demonstrate that two short-chain α-neurotoxins (SαNTx) functionally inhibit human muscle-type nAChR, but are markedly more reversible than against rat receptors. In contrast, two long-chain α-neurotoxins (LαNTx) show no species differences in potency or reversibility. Mutant studies identified two key residues accounting for this. Proteomic and clinical data suggest that paralysis in human snakebites is not associated with SαNTx, but with LαNTx, such as in cobras. Neuromuscular blockade produced by both subclasses of α-neurotoxins was reversed by antivenom in rat nerve-muscle preparations, supporting its effectiveness in human post-synaptic paralysis.

Language: en

Keywords

Neurotoxicity; Nicotinic acetylcholine receptor; Paralysis; Snakebite; α-Neurotoxins